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Our study still has deficiencies: (1) serum cytokines may not reflect renal inflammation, and spatial transcriptomics for mapping local inflammation in the future

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GHK-Cu's Documented Effects on TNF-α Published research has documented GHK-Cu's modulating effects on TNF-α in research models: Reductions in TNF-α expression in inflammatory tissue models Decreased TNF-α release from inflammatory cells in cell culture studies TNF-α modulation as part of GHK-Cu's broader gene expression effects (TNF-α gene expression is among the hundreds of genes documented in GHK-Cu microarray studies) Anti-inflammatory effects in wound healing and skin biology research contexts The TNF-α modulation appears to be part of GHK-Cu's broader pattern of gene expression effects rather than a single-target mechanism